Univ. of Manitoba SOP # VSP8

MEDIpoint, Inc.

MEDIpoint International, Inc.

SOP# VSP8

Veterinary Services - University of Manitoba

Revised/Reviewed: March 10, 2010

One Person Blood Collection from the Brachial Vein (Wing Vein) of Chickens

PURPOSE: To describe the methods for a one person blood collection technique from the brachial vein in chickens using the Goldenrod Animal Lancet.

 

RESPONSIBILITY: Technical personnel
SUPPLIES: blood collection tube, goldenrod lancet (4 mm), gauze, poultry crate

PROCEDURE: one person blood collection from the brachial vein of chickens
1. Prepare by setting poultry crate close to the location where the procedure is to be performed.
2. Have the lancet, blood collection tube and gauze ready for use.
3. Restrain the bird by holding it with the non-dominant hand around the base of the wing so that the middle finger and thumb almost touch.
4. Further restrain the bird by tucking its legs under the elbow of the non-dominant arm. Pressure from the forearm will keep the bird pressed against the restrainer’s chest. This will expose the wing web area and keep it on a horizontal plane to allow blood o pool in the depression.
5. Pluck a minimal amount of feathers over the elbow of the restrained wing to permit visualization of the brachial vein.
6. Puncture the vessel at a 900 angle with the lancet using your free dominant hand.
7. Collect the sample into the blood collection tube.
8. Apply pressure to the puncture site with gauze and maintain the pressure for approximately one minute.
9. Place the bird into the poultry crate and leave the bird for approximately 5 minutes or longer.
10. Return bird to its cage once confident the bleeding has stopped.
11. This bleeding technique is for the collection of small samples. However it must be noted that volumes drawn should not exceed 10% of the bird’s circulating blood volume. Blood volume as a percentage of body weight averages 7%.l

CLICK HERE TO SEE PICTURES OF THIS TECHNIQUE

References:
Murray Smith, On Farm Food Safety Inspector, Manitoba Agriculture, Food and Rural Initiatives.
MEDIpoint, Inc.: http://www.medipoint.com/html/for_use_on_chickens.htm

MEDIPOINT ACKNOWLEDGES THE FOLLOWING PEOPLE FOR THEIR HELP IN DEVELOPING AND REVISING THIS STANDARD OPERATING PROCEDURE:

Murray Smith, On Farm Food Safety Inspector, Manitoba Agriculture, Food and Rural Initiatives                                                       
Dr. Richard Hodges, Director, Animal Care and Use Program, Animal Care, VP Research, University of Manitoba                                  
Denise Borowski, Lab Animal Training Coordinator, Animal Care, VP Research, University of Manitoba                                                                
Heather Simpson, Animal Health Technician Practitioner, Animal Care, VP Research, University of Manitoba